hardshell 384 well plates Search Results


hardshell plate biorad  (Bio-Rad)
96
Bio-Rad hardshell plate biorad
Hardshell Plate Biorad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hardshell plate biorad/product/Bio-Rad
Average 96 stars, based on 1 article reviews
hardshell plate biorad - by Bioz Stars, 2026-02
96/100 stars
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twintec hardshell 384 well plates  (Eppendorf AG)
99
Eppendorf AG twintec hardshell 384 well plates
Twintec Hardshell 384 Well Plates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/twintec hardshell 384 well plates/product/Eppendorf AG
Average 99 stars, based on 1 article reviews
twintec hardshell 384 well plates - by Bioz Stars, 2026-02
99/100 stars
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384 well hardshell pcr plate  (Bio-Rad)
94
Bio-Rad 384 well hardshell pcr plate
( a ) Overview of the assay principle with live K562 cells seeded into a <t>384-well</t> <t>PCR</t> plate. The plate contains controls or library compounds that are taken up by the cells. Following a pre-incubation period the plate is transiently heated for 3 min followed by cooling and cell lysis. Part of the cell lysate is transferred to a detection plate, to which antibodies and AlphaScreen beads are added to allow measurements of remaining soluble TS. ( b ) CETSA derived T agg curves for TS in K562 cells in the presence of DMSO (0.5%) (green circle), 15 μM floxuridine (blue triangle) or 1 μM raltitrexed (magenta square). All data were normalized to the response observed for each treatment condition at the lowest test temperature. The solid line represents the best fit to the Boltzmann sigmoid equation resulting in an apparent T agg of 46.7±0.2 °C for the DMSO control, whereas both floxuridine and raltitrexed stabilized TS above 65 °C (we do not consider higher T agg values reliable as these temperatures influence cell membrane integrity ). The vertical dotted line is at 50 °C, the temperature selected for the isothermal screen. Data are provided as the average and standard error of mean (s.e.m.) from two independent experiments performed in duplicate for raltitrexed and as individual data points from one experiment in duplicate for floxuridine. ( c ) ITDRF CETSA of floxuridine (blue triangle) at 50 °C based on raw data from the AlphaScreen readings. The solid line represents the best fit to a saturation binding curve resulting in an EC 50 of 47±16 pM. Data are provided as two individual data points from one test occasion. ( d ) The corresponding ITDRF CETSA for raltitrexed (magenta square) at 50 °C resulting in an EC 50 of 0.75±0.2 nM. Data are provided as two individual data points from one test occasion.
384 Well Hardshell Pcr Plate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/384 well hardshell pcr plate/product/Bio-Rad
Average 94 stars, based on 1 article reviews
384 well hardshell pcr plate - by Bioz Stars, 2026-02
94/100 stars
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twintec hardshell 384-well plates  (Eppendorf AG)
90
Eppendorf AG twintec hardshell 384-well plates
( a ) Overview of the assay principle with live K562 cells seeded into a <t>384-well</t> <t>PCR</t> plate. The plate contains controls or library compounds that are taken up by the cells. Following a pre-incubation period the plate is transiently heated for 3 min followed by cooling and cell lysis. Part of the cell lysate is transferred to a detection plate, to which antibodies and AlphaScreen beads are added to allow measurements of remaining soluble TS. ( b ) CETSA derived T agg curves for TS in K562 cells in the presence of DMSO (0.5%) (green circle), 15 μM floxuridine (blue triangle) or 1 μM raltitrexed (magenta square). All data were normalized to the response observed for each treatment condition at the lowest test temperature. The solid line represents the best fit to the Boltzmann sigmoid equation resulting in an apparent T agg of 46.7±0.2 °C for the DMSO control, whereas both floxuridine and raltitrexed stabilized TS above 65 °C (we do not consider higher T agg values reliable as these temperatures influence cell membrane integrity ). The vertical dotted line is at 50 °C, the temperature selected for the isothermal screen. Data are provided as the average and standard error of mean (s.e.m.) from two independent experiments performed in duplicate for raltitrexed and as individual data points from one experiment in duplicate for floxuridine. ( c ) ITDRF CETSA of floxuridine (blue triangle) at 50 °C based on raw data from the AlphaScreen readings. The solid line represents the best fit to a saturation binding curve resulting in an EC 50 of 47±16 pM. Data are provided as two individual data points from one test occasion. ( d ) The corresponding ITDRF CETSA for raltitrexed (magenta square) at 50 °C resulting in an EC 50 of 0.75±0.2 nM. Data are provided as two individual data points from one test occasion.
Twintec Hardshell 384 Well Plates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/twintec hardshell 384-well plates/product/Eppendorf AG
Average 90 stars, based on 1 article reviews
twintec hardshell 384-well plates - by Bioz Stars, 2026-02
90/100 stars
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384 well hardshell plates  (Bio-Rad)
99
Bio-Rad 384 well hardshell plates
( a ) Overview of the assay principle with live K562 cells seeded into a <t>384-well</t> <t>PCR</t> plate. The plate contains controls or library compounds that are taken up by the cells. Following a pre-incubation period the plate is transiently heated for 3 min followed by cooling and cell lysis. Part of the cell lysate is transferred to a detection plate, to which antibodies and AlphaScreen beads are added to allow measurements of remaining soluble TS. ( b ) CETSA derived T agg curves for TS in K562 cells in the presence of DMSO (0.5%) (green circle), 15 μM floxuridine (blue triangle) or 1 μM raltitrexed (magenta square). All data were normalized to the response observed for each treatment condition at the lowest test temperature. The solid line represents the best fit to the Boltzmann sigmoid equation resulting in an apparent T agg of 46.7±0.2 °C for the DMSO control, whereas both floxuridine and raltitrexed stabilized TS above 65 °C (we do not consider higher T agg values reliable as these temperatures influence cell membrane integrity ). The vertical dotted line is at 50 °C, the temperature selected for the isothermal screen. Data are provided as the average and standard error of mean (s.e.m.) from two independent experiments performed in duplicate for raltitrexed and as individual data points from one experiment in duplicate for floxuridine. ( c ) ITDRF CETSA of floxuridine (blue triangle) at 50 °C based on raw data from the AlphaScreen readings. The solid line represents the best fit to a saturation binding curve resulting in an EC 50 of 47±16 pM. Data are provided as two individual data points from one test occasion. ( d ) The corresponding ITDRF CETSA for raltitrexed (magenta square) at 50 °C resulting in an EC 50 of 0.75±0.2 nM. Data are provided as two individual data points from one test occasion.
384 Well Hardshell Plates, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/384 well hardshell plates/product/Bio-Rad
Average 99 stars, based on 1 article reviews
384 well hardshell plates - by Bioz Stars, 2026-02
99/100 stars
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hardshell pcr plate 384 wells blue case of 50  (PerkinElmer)
N/A
Leveraging years of assay and instrument experience in plate detection PerkinElmer designs better microplates and microplate accessories for better performance that guarantees better results for all PerkinElmer applications
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( a ) Overview of the assay principle with live K562 cells seeded into a 384-well PCR plate. The plate contains controls or library compounds that are taken up by the cells. Following a pre-incubation period the plate is transiently heated for 3 min followed by cooling and cell lysis. Part of the cell lysate is transferred to a detection plate, to which antibodies and AlphaScreen beads are added to allow measurements of remaining soluble TS. ( b ) CETSA derived T agg curves for TS in K562 cells in the presence of DMSO (0.5%) (green circle), 15 μM floxuridine (blue triangle) or 1 μM raltitrexed (magenta square). All data were normalized to the response observed for each treatment condition at the lowest test temperature. The solid line represents the best fit to the Boltzmann sigmoid equation resulting in an apparent T agg of 46.7±0.2 °C for the DMSO control, whereas both floxuridine and raltitrexed stabilized TS above 65 °C (we do not consider higher T agg values reliable as these temperatures influence cell membrane integrity ). The vertical dotted line is at 50 °C, the temperature selected for the isothermal screen. Data are provided as the average and standard error of mean (s.e.m.) from two independent experiments performed in duplicate for raltitrexed and as individual data points from one experiment in duplicate for floxuridine. ( c ) ITDRF CETSA of floxuridine (blue triangle) at 50 °C based on raw data from the AlphaScreen readings. The solid line represents the best fit to a saturation binding curve resulting in an EC 50 of 47±16 pM. Data are provided as two individual data points from one test occasion. ( d ) The corresponding ITDRF CETSA for raltitrexed (magenta square) at 50 °C resulting in an EC 50 of 0.75±0.2 nM. Data are provided as two individual data points from one test occasion.

Journal: Nature Communications

Article Title: CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil

doi: 10.1038/ncomms11040

Figure Lengend Snippet: ( a ) Overview of the assay principle with live K562 cells seeded into a 384-well PCR plate. The plate contains controls or library compounds that are taken up by the cells. Following a pre-incubation period the plate is transiently heated for 3 min followed by cooling and cell lysis. Part of the cell lysate is transferred to a detection plate, to which antibodies and AlphaScreen beads are added to allow measurements of remaining soluble TS. ( b ) CETSA derived T agg curves for TS in K562 cells in the presence of DMSO (0.5%) (green circle), 15 μM floxuridine (blue triangle) or 1 μM raltitrexed (magenta square). All data were normalized to the response observed for each treatment condition at the lowest test temperature. The solid line represents the best fit to the Boltzmann sigmoid equation resulting in an apparent T agg of 46.7±0.2 °C for the DMSO control, whereas both floxuridine and raltitrexed stabilized TS above 65 °C (we do not consider higher T agg values reliable as these temperatures influence cell membrane integrity ). The vertical dotted line is at 50 °C, the temperature selected for the isothermal screen. Data are provided as the average and standard error of mean (s.e.m.) from two independent experiments performed in duplicate for raltitrexed and as individual data points from one experiment in duplicate for floxuridine. ( c ) ITDRF CETSA of floxuridine (blue triangle) at 50 °C based on raw data from the AlphaScreen readings. The solid line represents the best fit to a saturation binding curve resulting in an EC 50 of 47±16 pM. Data are provided as two individual data points from one test occasion. ( d ) The corresponding ITDRF CETSA for raltitrexed (magenta square) at 50 °C resulting in an EC 50 of 0.75±0.2 nM. Data are provided as two individual data points from one test occasion.

Article Snippet: Finally 5 μl of the diluted compounds were transferred to a 384 well hardshell PCR plate (HSR480, BIORAD) using a Bravo liquid handling platform equipped with a 384-well head (Agilent).

Techniques: Incubation, Lysis, Amplified Luminescent Proximity Homogenous Assay, Derivative Assay, Control, Membrane, Binding Assay